KRT20
Reaktivität: Human
IHC, StM
Wirt: Maus
Monoclonal
KRT20-3145
unconjugated
Applikationshinweise
Immunohistochemistry on Paraffin Sections: Use a dilution of 1/25-1/50 in an ABC method(30 minutes at room temperature). Formalin fixed paraffin embedded tissue sectionsrequire high temperature antigen unmasking with 10 mM Citrate buffer, pH 6.0 prior toimmunostaining. Positive Control: Skin. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Cytokeratin 20 is a 46 kDa intermediate filament protein whose expression is restricted primarily to gastric and intestinal epithelium, urothelium, and Merkel cells. This antibody is highly specific to cytokeratin 20 and shows no cross-reactivity with other IFPs. Cytokeratin 20 is a unique type I keratin that is expressed in adenocarcinomas of the colon stomach, pancreas and bile system. It is also expressed in mucinous ovarian tumors, transitional cell carcinomas of the urinary tract, and Merkel cell carcinomas. This antibody is essentially non-reactive in squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas. This antibody is often used in conjunction with CK7 and other antibodies to distinguishing colon carcinomas (CK20+), from ovarian, pulmonary, and breast carcinomas.Synonyms: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT