SAA Antikörper

Produktnummer ABIN114511

Dieser Maus Monoklonal Antikörper detektiert spezifisch SAA in WB, EIA und ELISA (Capture). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für SAA Antikörper (ABIN114511)

Target: SAA (Serum Amyloid A (SAA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SAA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), ELISA (Capture)

Klon: 115

Produktdetails anti-SAA Antikörper

Spezifität: This monoclonal antibody reacts with natural and recombinant SAA. Does not show any cross-reaction with other human cytokines or growth factors tested such as IL-1 beta, IL-8, MCAF, TGF-beta and EGF.

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Highly purified recombinant human SAA (MW: 12 kDa)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: This antibody can be used as a capture antibody in sandwich ELISA applications forhuman SAA detection in combination with a monoclonal tracer/detection antibody(HRPconjugated, Cat. No. : ABIN114514). Suggested Capture Coating Dose: 0.3 g/well, Substrate: TMB. If the above suggestedconditions are followed, approximately 1.1 ng/mL of SAA in serum/plasma or 0.6 ng/mL ofSAA in medium can be detected with an assay range of 0 to 80 ng/mL. Western Blot: An antibody concentration of 0.1-0.5 g will allow visualization of 100 ng/lane of recombinant human SAA.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Double distllated water is recommended to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu SAA

Target: SAA (Serum Amyloid A (SAA))

Andere Bezeichnung: Serum Amyloid A Protein (SAA)

Hintergrund: The serum amyloid A (SAA) family comprises a number of differentially expressed lipoproteins, acute phase SAA1 and SAA2, the former being a major component in plasma, and constitutive SAA's (C-SAAs). Although the liver is the primary site of synthesis of both SAA types, extrhepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed it's importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations. SAA rises earlier and more sharply than CRP. SAA enhances the binding of HDL's to macrophages and thus helps the delivery of lipid to sites of injury for use in tissue repair. It is thus thought to be an integral part of the disease process. In addition, recent experiments suggest that SAA may play a "houekeeping" role in normal human tissues. Elevated levels of SAA over time predispose secondary amyloidosis, extracellular accumulation of amyloid fibrils, derived from a circulating precursor, in various tissues and organs. The most common form of amyloidosis occurs secondary to chronic inflammatory disease, particularly rheumatoid artheritis.Synonyms: SAA1, SAA2

Gen-ID: 6289, 9606

UniProt: P02735