Macrophages Antikörper

Produktnummer ABIN114398

Der Kaninchen Polyklonal anti-Macrophages Antikörper wird verwendet zum Nachweis von Macrophages in Proben von Maus. Er wurde validiert für IHC (fro), IHC (p) und Func.

Kurzübersicht für Macrophages Antikörper (ABIN114398)

Target: Macrophages

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Produktdetails

Aufreinigung: Affinity Purified

Anwendungsinformationen

Applikationshinweise: Cytotoxic Assays. Immunohistochemistry on Frozen and Paraffin Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Test Type: For Cytotoxic Antibodies: Modified Colorimetric Microtiter Assay (Ref. 1)Results: Antisera of this lot diluted 1/20 exhibits greater than 75% cytotoxicity on MouseMacrophages. Antisera of this lot diluted 1/20 exhibits less than 10% cytotoxicity on Mouse thymocytes orsplenocytes. For Agglutinating Antibodies: Antisera dilutions in RPMI-1640 incubated with target cells at 4-8°C for 1hr. Agglutinationdetermined by microscopic observations. Results: Antisera of this lot strongly agglutinates mouse macrophages at a dilution of 1/50.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Antigendetails

Target: Macrophages

Hintergrund: Synonyms: Macrophage marker