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MHC Class I (RT1Ac) Antikörper (Biotin)

Reaktivität: Ratte FACS, IHC (fro) Wirt: Maus Monoclonal OX-27 Biotin
Produktnummer ABIN114391
$387.20
Zzgl. Versandkosten $45.00
0.1 mg
Available
  • Target See all MHC Class I (RT1Ac) products
    MHC Class I (RT1Ac)
    Reaktivität
    Ratte
    Wirt
    • 7
    Maus
    Klonalität
    • 7
    Monoklonal
    Konjugat
    • 3
    • 2
    • 1
    • 1
    Dieser MHC Class I (RT1Ac) Antikörper ist konjugiert mit Biotin
    Applikation
    • 7
    • 2
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Aufreinigung
    Protein A Chromatography
    Immunogen
    Phytohaemagglutinin Blasts Donor: BALB/c spleen Fusion Partner: P3-NS1-1-Ag4 (NS1/1)
    Klon
    OX-27
    Isotyp
    IgG2a
  • Applikationshinweise
    Flow Cytometry. Immunohistochemistry using cryostat sections: (however, cross-reactivity with Lewis ratshave been shown to occur in some instances).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protokoll
    FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-Rat cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 5-1. 0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1: 500 dilution. Notrecommended for use with a PE secondary. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Rat Strain: Brown NorwayCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1. 0 µg/10e6 cellsIsotypic Control: Biotin Mouse IgG2aCell Source Percentage of cells stained above control: Thymus 65. 4%Spleen 98. 9%Lymph Node 100%Restults - Strain Distribution: Cell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1. 0 µg/10e6 cellsStrains Tested: Brown Norway, Buffalo, Wistar, Fischer, ACIPositive: Brown NorwayNegative: Buffalo, Wistar, Fischer, ACI
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.1 mg/mL
    Buffer
    PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    MHC Class I (RT1Ac)
    Abstract
    MHC Class I (RT1Ac) Produkte
    Hintergrund
    MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: MHC class I RT1.Ac heavy chain, RT1-A3
    Gen-ID
    10116
    UniProt
    Q31255
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