MHC Class II (RT1D) Antikörper

Produktnummer ABIN114389

Der Maus Monoklonal anti-MHC Class II (RT1D) Antikörper wird verwendet zum Nachweis von MHC Class II (RT1D) in Proben von Ratte. Er wurde validiert für FACS, IHC (fro) und IP.

Kurzübersicht für MHC Class II (RT1D) Antikörper (ABIN114389)

Target: MHC Class II (RT1D)

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Klon: OX-17

Produktdetails

Aufreinigung: Protein G affinity chromatography

Immunogen: Rat spleen membrane glycoproteins depleted of Ia-A antigens Immunocyte Donor: BALB/c spleen Fusion Partner: X63 Ag8.653

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 1/250 - 1/500 (see Protocols). Immunhistochemistry. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: FLOW CYTOMETRY ANALYSIS: METHOD: 1. Prepare cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-Rat. cell separation medium. 2. Wash 2 times. 3. Resuspend cells to 1x10e6 cells in approximately 50 µl media A in a microcentrifugetube. (i. e. 50 µl of cells resuspended to 2x10e7 cells/ml). The contents of 1 tube represent 1test. 4. To each tube add 50 µl of a 1: 250 - 1: 500 dilution of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-mouse IgG (H+L)) at 1: 700 dilution. 9. Incubate tubes at 4°C for 30-60 minutes. (It is recommended that the tubes areprotected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in Media B. 11. Resuspend the cell pellet in 50 µl ice cold Media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in phosphate buffered saline. (This stains dead cells by intercalatingDNA). MEDIA: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2 M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% bovine serum albumin + sodium azide (100 µlof 2 M sodium azide in 100 mls). RESULTS: Rat Strain: Lewis RatCell Concentration: 1x10e6 cells per testAntibody Concentration: 1: 400Isotypic Control: Mouse IgG1, κCELL SOURCE PERCENT STAININGThymus 12%Spleen 34%Lymph Node 25%STRAIN DISTRIBUTION: Antibody Concentration: 1: 100Strains Tested: Wistar, Buffalo, Brown Norway, Fischer 344Positive: Wistar, Buffalo, Brown Norway, ACI, Fischer 344Negative: none

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS with 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Antigendetails

Target: MHC Class II (RT1D)

Andere Bezeichnung: MHC Class II RT1D

Hintergrund: Synonyms: HLA Class II