MHC Class II I-Ek Antikörper
Kurzübersicht für MHC Class II I-Ek Antikörper (ABIN114319)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Aufreinigung
- Protein G chromatography
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Immunogen
- C3H Donor: C3H.SW Fusion Partner: SP2/0-Ag 14
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Isotyp
- IgG2a
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Applikationshinweise
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Cytotoxicity analysis.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Protokoll
- CYTOTOXICITY ANALYSIS: Method: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Mediuma orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on Lympholyte®-Mb cell separation medium. After washing, adjust the cellconcentration to 1x10e6 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1: 1000 and mix.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 1.0 mg/mL
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Buffer
- PBS and 0.02 % NaN3
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Avoid repeated freezing and thawing.
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Lagerung
- 4 °C/-20 °C
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Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MHC Class II I-Ek
Target
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