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MHC Class II I-Ap Antikörper

Reaktivität: Maus FACS, CyTox Wirt: Maus Monoclonal 7-16-17 unconjugated
Produktnummer ABIN114314
  • Target
    MHC Class II I-Ap
    Reaktivität
    Maus
    Wirt
    • 5
    Maus
    Klonalität
    • 5
    Monoklonal
    Konjugat
    • 2
    • 1
    • 1
    • 1
    Unkonjugiert
    Applikation
    Flow Cytometry (FACS), Cytotoxicity Test (CyTox)
    Aufreinigung
    Protein G chromatography
    Immunogen
    B10.p
    Klon
    7-16-17
    Isotyp
    IgG2a
  • Applikationshinweise
    Flow cytometry (protocol see below). Cytotoxic assay.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protokoll
    FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 2-1. 0 µg of antibody. 5. Vortex the tubes to ensure thorough mixing of antibody and cells.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.0 mg/mL
    Buffer
    PBS and 0.02 % sodium azide (NaN3).
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing. Dilute only prior to immediate use
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    MHC Class II I-Ap
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