Flow cytometry (protocol see below). Cytotoxic assay. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protokoll
FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 2-1. 0 µg of antibody. 5. Vortex the tubes to ensure thorough mixing of antibody and cells.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS and 0.02 % sodium azide (NaN3).
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.