MHC Class II I-Ap Antikörper

Produktnummer ABIN114314

Der Maus Monoklonal anti-MHC Class II I-Ap Antikörper wird verwendet zum Nachweis von MHC Class II I-Ap in Proben von Maus. Er wurde validiert für FACS und CyTox.

Kurzübersicht für MHC Class II I-Ap Antikörper (ABIN114314)

Target: MHC Class II I-Ap

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Cytotoxicity Test (CyTox)

Klon: 7-16-17

Produktdetails

Aufreinigung: Protein G chromatography

Immunogen: B10.p

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry (protocol see below). Cytotoxic assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 2-1. 0 µg of antibody. 5. Vortex the tubes to ensure thorough mixing of antibody and cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS and 0.02 % sodium azide (NaN3).

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Antigendetails

Target: MHC Class II I-Ap