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CD90 Antikörper (PE)

THY1 Reaktivität: Maus FACS Wirt: Maus Monoclonal 5a-8 PE
Produktnummer ABIN114255
  • Target Alle CD90 (THY1) Antikörper anzeigen
    CD90 (THY1)
    Reaktivität
    • 146
    • 134
    • 63
    • 32
    • 27
    • 23
    • 22
    • 16
    • 7
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 3
    Maus
    Wirt
    • 112
    • 84
    • 72
    • 1
    Maus
    Klonalität
    • 193
    • 76
    Monoklonal
    Konjugat
    • 102
    • 33
    • 21
    • 21
    • 17
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD90 Antikörper ist konjugiert mit PE
    Applikation
    • 180
    • 90
    • 58
    • 44
    • 31
    • 30
    • 28
    • 26
    • 14
    • 13
    • 13
    • 9
    • 9
    • 7
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    Flow Cytometry (FACS)
    Spezifität
    This monoclonal antibody reacts with all T lymphocytes from mouse strains expressing the Thy 1.2 phenotype (e.g. C57BL/6, C3H/He, DBA/2, CBA/J, BALB/c), but does not react with lymphocytes expressing the Thy 1.1 phenotype [e.g. AKR/J, B6.PL(74NS)].
    Aufreinigung
    Protein G Chromatography.
    Immunogen
    CBA/J. Donor: AKR/J Spleen. Fusion Partner: Spleen from immunized recipient fused with myeloma P3-NSI-1-Ag4-1.
    Klon
    5a-8
    Isotyp
    IgG2b
    Top Product
    Discover our top product THY1 Primärantikörper
  • Applikationshinweise
    Flow Cytometry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protokoll
    FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 5 µg* of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 5 µg/10e6 cellsIsotypic Control: PE-Mouse IgG2b,kCell Source Percentage of cells stained above control: Thymus: 98. 4%Spleen: 28. 8%Results - Strain Distribution: Cell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 5 µg/10e6 cellsStrains Tested: C57BL/6, C3H/He, CBA/J, BALB/c, ATL, AKR/JPositive: C57BL/6, C3H/He, CBA/J, BALB/c, ATLNegative: AKR/J
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.1 mg/mL
    Buffer
    PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    This product is photosensitive and should be protected from light
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
  • Target
    CD90 (THY1)
    Andere Bezeichnung
    CD90 (THY1 Produkte)
    Synonyme
    CD90 antikoerper, CD7 antikoerper, T25 antikoerper, Thy-1 antikoerper, Thy-1.2 antikoerper, Thy1.1 antikoerper, Thy1.2 antikoerper, Thy-1 cell surface antigen antikoerper, thymus cell antigen 1, theta antikoerper, THY1 antikoerper, Thy1 antikoerper
    Hintergrund
    CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein
    Gen-ID
    21838
    NCBI Accession
    NP_033408
    UniProt
    P01831
    Pathways
    Cell-Cell Junction Organization
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