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CD90 Antikörper (FITC)

THY1 Reaktivität: Maus FACS Wirt: Maus Monoclonal 5a-8 FITC
Produktnummer ABIN114252
  • Target Alle CD90 (THY1) Antikörper anzeigen
    CD90 (THY1)
    Reaktivität
    • 146
    • 134
    • 63
    • 32
    • 27
    • 23
    • 22
    • 16
    • 7
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 3
    Maus
    Wirt
    • 112
    • 84
    • 72
    • 1
    Maus
    Klonalität
    • 193
    • 76
    Monoklonal
    Konjugat
    • 102
    • 33
    • 21
    • 21
    • 17
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD90 Antikörper ist konjugiert mit FITC
    Applikation
    • 180
    • 90
    • 58
    • 44
    • 31
    • 30
    • 28
    • 26
    • 14
    • 13
    • 13
    • 9
    • 9
    • 7
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    Flow Cytometry (FACS)
    Spezifität
    This monoclonal antibody reacts with all T lymphocytes from mouse strains expressing the Thy 1.2 phenotype (e.g. C57BL/6, C3H/He, DBA/2, CBA/J, BALB/c), but does not react with lymphocytes expressing the Thy 1.1 phenotype [e.g. AKR/J, B6.PL(74NS)].
    Kreuzreaktivität (Details)
    Species reactivity (tested):Mouse.
    Aufreinigung
    Protein G Chromatography.
    Immunogen
    CBA/J. Donor: AKR/J Spleen. Fusion Partner: Spleen from immunized recipient fused with myeloma P3-NSI-1-Ag4-1.
    Klon
    5a-8
    Isotyp
    IgG2b
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    Discover our top product THY1 Primärantikörper
  • Applikationshinweise
    Flow Cytometry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protokoll
    FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 1/50 -1/100 dilution in 50 µl of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive)7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testsAntibody Concentration Used: 1/50 in 50 µl/10e6 cellsIsotypic Control: FITC Mouse IgG2bCell Source Percentage of cells stained above control: Thymus: 97. 4%Spleen: 52. 6%Results - Strain Distribution: Tissue: SpleenCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1/100 in 50 µl/10e6 cellsStrains Tested: C57BL/6, C3H/He, CBA/J, BALB/c, ATL, AKR/JPositive: C57BL/6, C3H/He, CBA/J, BALB/c, ATLNegative: AKR
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.1 mg/mL
    Buffer
    PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    CD90 (THY1)
    Andere Bezeichnung
    CD90 (THY1 Produkte)
    Synonyme
    CD90 antikoerper, CD7 antikoerper, T25 antikoerper, Thy-1 antikoerper, Thy-1.2 antikoerper, Thy1.1 antikoerper, Thy1.2 antikoerper, Thy-1 cell surface antigen antikoerper, thymus cell antigen 1, theta antikoerper, THY1 antikoerper, Thy1 antikoerper
    Hintergrund
    CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein
    Gen-ID
    21838
    NCBI Accession
    NP_033408
    UniProt
    P01831
    Pathways
    Cell-Cell Junction Organization
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