Fibronectin Antikörper

Produktnummer ABIN113992

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Fibronectin in IF, IHC (p), EIA, IHC (fro) und RIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Fibronectin Antikörper (ABIN113992)

Target: Fibronectin

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Fibronectin Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Produktdetails anti-Fibronectin Antikörper

Spezifität: This antibody is specific for Fibronectin (100 %). Collagen type I, III, IV and VI < 0.1 % (RIA at 1/10000 dilution). This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.

Aufreinigung: Affinity Chromatography.

Immunogen: Purified fibronectin from human plasma

Anwendungsinformationen

Applikationshinweise: Immunofluorescence: 1/80. Immunohistochemistry: 1/200-1/600 for Paraffin Sections, 60 min at RT or 2-8 °C overnight. ELISA (> / = 1/200). RIA. Positive Control: Human skin. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered solution without BSA or preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Fibronectin

Target: Fibronectin

Hintergrund: Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.Synonyms: CIG, Cold-insoluble globulin, FN1

Gen-ID: 2335

NCBI Accession: NP_002017

UniProt: P02751