Elastin Antikörper

Produktnummer ABIN113991

Dieses Anti-Elastin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Elastin in IHC (p), IF, RIA, IHC (fro) und EIA. Geeignet für Human.

Kurzübersicht für Elastin Antikörper (ABIN113991)

Target: Elastin (ELN)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Elastin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Produktdetails anti-Elastin Antikörper

Spezifität: This antibody is specific for Human elastin. Cross-reactivity in RIA at 1:500 dilution: human collagen type I, II, III, IV, V < 0.1 %, human fibronectin < 0.1 % . Cross reaction with rat < 0.1 %.

Aufreinigung: Affinity chromatography

Immunogen: Purified elastin from human skin

Anwendungsinformationen

Applikationshinweise: IF: 1: 80. IHC: 1: 500 for paraffin sections, 60 min at RT or 2-8C over night. ELISA: > 1: 200. RIA. Positive control: Human skin or aorta. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Reconstitute with 0.5 mL distilled water. For further dilution use appropriate antibody diluent.

Konzentration: App. 1 mg/mL

Buffer: Phosphate buffered solution, no BSA and preservative added

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Elastin

Target: Elastin (ELN)

Andere Bezeichnung: Elastin

Hintergrund: Elastin is the major protein of elastic fibres that form a randomly orientated network in many tissues. Due to its high content of hydrophobic amino acids it is relatively resistant to chemicals and proteinase. Its principal function is to provide elasticity to tissues, however it has also chemotactic and cell adhesion properties.Synonyms: ELN, Tropoelastin

Gen-ID: 2006

NCBI Accession: NP_000492

UniProt: P15502

Pathways: Regulation of Actin Filament Polymerization