Fibronectin Antikörper

Produktnummer ABIN113981

Der Kaninchen Polyklonal Anti-Fibronectin-Antikörper wurde für IHC (p), EIA, IHC (fro) und RIA validiert. Er ist geeignet, Fibronectin in Proben von Maus zu detektieren.

Kurzübersicht für Fibronectin Antikörper (ABIN113981)

Target: Fibronectin

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Fibronectin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Produktdetails anti-Fibronectin Antikörper

Kreuzreaktivität (Details): Species reactivity (tested):Mouse.

Aufreinigung: Affinity Chromatography.

Immunogen: Purified fibronectin from Murine plasma.

Anwendungsinformationen

Applikationshinweise: This antibody can be used on Frozen and Paraffin Embedded Tissues: IFA (1/80), IHC(P)(1/500) After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blockingserum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBSfor 30 min at RT is recommended. Incubation time: IHC(P) 60 min at RT or 2-8 °C over night. Also suitable for ELISA (1/200) and RIA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water, dilute further with appropriate antibody diluent.

Konzentration: 1.0 mg/mL

Buffer: PBS without preservative or stabilizer.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Fibronectin

Target: Fibronectin

Hintergrund: Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.Synonyms: CIG, Cold-insoluble globulin, FN1

Gen-ID: 10090

UniProt: P11276