Fibronectin Antikörper
Kurzübersicht für Fibronectin Antikörper (ABIN113980)
Target
Alle Fibronectin Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Aufreinigung
- Affinity Chromatography.
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Immunogen
- Purified Fibronectin from Rat plasma.
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Applikationshinweise
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Immunohistochemistry on Paraffin Sections: 1/200-1/600. Immunofluorescence: 1/80. Incubation Time: 60 min at RT or 2-8 °C over night. Positive Control: Rat Skin, Liver. Pretreatment: After de-waxing the tissue slices are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blockingserum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBSfor 30 min at RT is recommended. Also suitable for use in RIA and ELISA: >/= 1/200 (OD >/=500).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Rekonstitution
- Restore with 0.5 mL distilled water.
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Konzentration
- 1.0 mg/mL
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Buffer
- PBS without BSA or preservatives.
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Konservierungsmittel
- Without preservative
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store at 2-8 °C.
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- Fibronectin
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Hintergrund
- Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumors.Synonyms: CIG, Cold-insoluble globulin, FN1
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Gen-ID
- 10116
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UniProt
- P04937
Target
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