Fibronectin Antikörper

Produktnummer ABIN113978

Der Kaninchen Polyklonal Anti-Fibronectin-Antikörper wurde für IHC (p), EIA, IHC (fro) und RIA validiert. Er ist geeignet, Fibronectin in Proben von Rind (Kuh) zu detektieren.

Kurzübersicht für Fibronectin Antikörper (ABIN113978)

Target: Fibronectin

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Fibronectin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Produktdetails anti-Fibronectin Antikörper

Aufreinigung: Purified

Immunogen: Purified fibronectin from bovine plasma

Anwendungsinformationen

Applikationshinweise: IF: 1: 40. IHC: 1: 200 - 1: 600 for paraffin sections, 60 min at RT or 2-8C over night. ELISA. RIA. Positive control: Bovine skin. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Reconstitute with 0.5 mL distilled water, for further dilution use appropriate antibody diluent.

Konzentration: App. 1 mg/mL

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Fibronectin

Target: Fibronectin

Hintergrund: Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.Synonyms: CIG, Cold-insoluble globulin, FN1

Gen-ID: 9913

UniProt: P07589