Der Ziege Polyklonal anti-Lipopolysaccharides (LPS) Antikörper (ABIN113127) detektiert spezifisch Lipopolysaccharides (LPS) in IF.
Dieser Antikörper reagiert spezifisch mit Proben aus Gram-negative Bakterien.
Indirect Immunofluoresence: > 1/100 (against members of the Entero-bacteriaciae). May also be used in place of neat antiserum in almost any appropriate antibody-basedtechnique. Also suitable for conjugation purposes. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
4-5 mg/mL (OD280 nm, E0.1% = 1.4)
Buffer
0.01 M PBS, pH 7.2, 0.09 % Sodium Azide, None
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
Lipopolysaccharides (LPS)
Substanzklasse
Chemical
Hintergrund
Lipid A is a lipid component of an endotoxin held responsible for toxicity of Gram-negative bacteria. Sensing of lipid A by the human immune system may also be critical for the onset of immune responses to Gram-negative infection, and for the subsequent successful fight against the infection. Lipid A is located at one end of the lipopolysaccharide (LPS, also called endotoxin) molecule, and anchors the LPS to the outer membrane of a Gram-negative bacteria. Many of the immune activating abilities of LPS can be contributed to the lipid A unit. It is a very potent stimulant of the immune system, activating cells (for example, monocytes or macrophages) at picogram per milliliter quantities. When present in the body at high concentrations during a Gram-negative bacterial infection, it may cause shock and death by an "out of control" excessive immune reaction.Synonyms: LPS, Lipid A