Topoisomerase I (TOP1)
(Topoisomerase (DNA) I (TOP1))
Reaktivität
Human
Wirt
Human
Klonalität
Polyklonal
Konjugat
Dieser Topoisomerase I Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), Immunodiffusion (ID), Enzyme Immunoassay (EIA)
Spezifität
Shows only the Scl-70 precipitate band when tested using double immunodiffusion against rabbit thymus extract. Specificity is verified by identity of precipitation with CDC-AF reference serum. The titer obtained will vary with the test system, however, undiluted antibody shows a strong Scl-70 precipitate band. Product shows no reactivity at a dilution of 1:100 when tested against purified SSA, SSB, Sm, Sm/RNp and Jo-1 antigen in an ELISA system designed to detect all IgG subclasses.
Aufreinigung
Precipitation of Ig fraction from delipidized and defibrinated human plasma.
Suitable for use in Immunodiffusion and ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Reconstitute with 2 mL deionized water.
Buffer
0.01 M PBS, 0.13 M NaCl containing 0.02 % Sodium azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
Do Not Add Sodium Azide.
Handhabung
Avoid repeated freezing and thawing. Dilute only prior to immediate use.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store vial at 2-8 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 °C or below.
Target
Topoisomerase I (TOP1)
(Topoisomerase (DNA) I (TOP1))