CHRM3 Antikörper (AA 580-589)

Produktnummer ABIN112905

Dieses Anti-CHRM3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CHRM3 in WB und IHC (fro). Geeignet für Human und Ratte.

Kurzübersicht für CHRM3 Antikörper (AA 580-589) (ABIN112905)

Target: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHRM3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-CHRM3 Antikörper

Bindungsspezifität: AA 580-589

Spezifität: Specific for the C-term of the M3 receptor (580-589): 100 % . Crossreactivity to full receptor 70 %, M1 (451-460), M2 (457-466), M4 (469-478) and M5 (519-531) < 0.01 %.

Immunogen: Synthetic peptide M3 (580-589) covalently attached to a carrier protein.

Anwendungsinformationen

Applikationshinweise: Immunocytochemical: this product has been found to stain PLP fixed rat brain sectionsknown to express the muscarinic M3 receptor using ABC techniques at a dilution of1: 2,500. Western immunoblotting: successful by using whole rat brain homogenerate at a dilutionof 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 0.1 mL PBS containing 10 mg/mL BSA or with additional buffer for more dilute antibody.

Konservierungsmittel: Azide free

Vorsichtsmaßnahmen: Do Not Use Sodium Azide as preservative.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Details zu CHRM3

Target: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Andere Bezeichnung: Muscarinic Acetylcholine Receptor M3

Hintergrund: This receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Expression of this receptor has been reported in brain, eye, and heart. ESTs have been isolated from brain, colon, fetus, lung, and prostate libraries.Synonyms: CHRM3

Gen-ID: 1131

NCBI Accession: NP_000731

UniProt: P20309

Pathways: Synaptic Membrane