M13 Bacteriophage G8P Capsid Antikörper

Produktnummer ABIN112403

Der Maus Monoklonal Anti--Antikörper wurde für WB, EIA, FACS, IHC (fro) und IF validiert. Er ist geeignet, in Proben von Bacteriophage M13 zu detektieren.

Kurzübersicht für M13 Bacteriophage G8P Capsid Antikörper (ABIN112403)

Target: M13 Bacteriophage G8P Capsid

Reaktivität: Bacteriophage M13

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Klon: RL-ph2

Produktdetails

Spezifität: RL-ph2 reacts with the M13 filamentous phage coat protein g8p with a Molecular Weight of 5 kDa.

Aufreinigung: Affinity Chromatography.

Immunogen: Isolated M13 phage coat proteins. Remarks: Derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: RL-ph2 is particularly useful for immunoblotting of separated phage proteins, and is alsosuitable for detection of M13 phages in multistep immunoblotting procedures,immunocytochemistry, flow cytometry, affinity chromatography and ELISA. Optimalantibody dilution should be determined by titration, recommended range is 1: 25 - 1: 200 forflow cytometry, and for immunohistochemistry with avidin-biotinylated horseradishperoxidase complex (ABC) as detection reagent, and 1: 100 - 1: 1000 for immunoblottingapplications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store lyophilized at 2-8 °C. After reconstitution aliquot and store at -20 °C.

Antigendetails

Target: M13 Bacteriophage G8P Capsid

Substanzklasse: Phage Protein

Hintergrund: The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.Synonyms: Coat protein B, Gene 8 protein, Major coat protein

Gen-ID: 927333

NCBI Accession: NP_510890

UniProt: P69541