Cardiotin Antikörper

Produktnummer ABIN112391

Der Maus Monoklonal Anti--Antikörper wurde für WB, IHC (fro) und IHC (p) validiert. Er ist geeignet, in Proben von Human, Schwein, Cat, Hund, Ziege, Hamster, Affe, Maus, Ratte, Xenopus laevis und Zebrafisch (Danio rerio) zu detektieren.

Kurzübersicht für Cardiotin Antikörper (ABIN112391)

Target: Cardiotin

Reaktivität: Human, Schwein, Cat, Hund, Ziege, Hamster, Affe, Maus, Ratte, Xenopus laevis, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: R2G

Produktdetails

Spezifität: R2G recognizes the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits. This antibody reacts with Canine, Feline, Goat, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Xenopus, Zebrafish tissues.

Aufreinigung: Purified

Immunogen: Total protein extract of Chicken gizzard.

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: This antibody is suitable for Immunohistochemistry on frozen and paraffin-embeddedtissue and Immunoblotting. Recommended dilutions: 1/25-1/100 for immunohistochemistry with avidinbiotinylatedhorseradish peroxidase complex (ABC)as detection reagent.1/25-1/500 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS with 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the lyophilized antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. When reconstituted the antibody is stable for one month at 2-8 °C.

Antigendetails

Target: Cardiotin

Hintergrund: Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle α-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.