Reticulon 1 Antikörper
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- Target Alle Reticulon 1 (RTN1) Antikörper anzeigen
- Reticulon 1 (RTN1)
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Reaktivität
- Human, Kaninchen, Affe
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Reticulon 1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- Spezifität
- RNL-2 recognizes an epitope located within the region of amino acids 421-589 of the neuroendocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B). In normal tissues, RNL-2 reacts with brain Purkinje cells, pancreatic islet cells, cells in the pituitary gland and some (peripheral) nerve fibers. In addition, a few epithelia show positive staining.
- Kreuzreaktivität (Details)
- Species reactivity (tested):Human, Rabbit and Rhesus Monkey.
- Aufreinigung
- Purified
- Immunogen
- The small cell lung cancer cell line NCI-H82.
- Klon
- RNL-2
- Isotyp
- IgG1
- Top Product
- Discover our top product RTN1 Primärantikörper
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- Applikationshinweise
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Suitable for Immunocytochemistry, Immunohistochemistry on frozen tissues,Immunoblotting and Flow cytometry. Recommended dilutions: 1/25-1/200 for Flow cytometry, and for Immunohistochemistry with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent.1/100-1/1000 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- PBS, 0.09 % Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store the antibody undiluted -20 °C only.
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- Target
- Reticulon 1 (RTN1)
- Andere Bezeichnung
- Reticulon 1 / RTN1 (RTN1 Produkte)
- Synonyme
- NSP antikoerper, 0710005K15Rik antikoerper, 4930441F12Rik antikoerper, Nsp antikoerper, R75279 antikoerper, Rtn1-a antikoerper, Rtn1-b antikoerper, Rtn1-c antikoerper, reticulon-1 antikoerper, RTN1 antikoerper, RTN1-Cw antikoerper, rtn1 antikoerper, XRTN1-A antikoerper, XRTN1-C.1 antikoerper, xrtn1 antikoerper, xrtn1-c antikoerper, RTN1.2 antikoerper, Rtn1-C.2 antikoerper, rtn1-a antikoerper, rtn1b antikoerper, xRTN1 antikoerper, RTN1.1 antikoerper, rtn1a antikoerper, xrtn1-c.1 antikoerper, rtn1l antikoerper, wu:fa21b09 antikoerper, wu:fj35h01 antikoerper, reticulon 1 antikoerper, reticulon-1 antikoerper, reticulon 1 S homeolog antikoerper, reticulon 1 L homeolog antikoerper, reticulon 1b antikoerper, RTN1 antikoerper, Rtn1 antikoerper, rtn1 antikoerper, Rtnl1 antikoerper, LOC100580843 antikoerper, rtn1.S antikoerper, rtn1.L antikoerper, LOC100339591 antikoerper, rtn1b antikoerper
- Hintergrund
- Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSPA), Reticulon-1B (NSP-B), and Reticulon-1C (NSPC). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).Synonyms: NSP, Neuroendocrine-specific protein, RTN1, Reticulon-1
- Gen-ID
- 6252
- NCBI Accession
- NP_066959
- UniProt
- Q16799
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