Selectin E/CD62e Antikörper

Produktnummer ABIN112381

Produktdetails anti-Selectin E/CD62e Antikörper

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Selectin E/CD62e Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Spezifität: UZ6 recognizes a conformational epitope in the CR2 region in murine E-selectin. UZ6 partially inhibits leukocyte adhesion to murine activated endothelial cells.

Aufreinigung: Purified

Immunogen: LPS-activated mlEND1 cells expressing E-selectin at their cell surface.

Klon: UZ6

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: This antibody is suitable for Immunoblotting, ELISA, Immunoprecipitation,Immunohistochemistry on fozen sections and Flow cytometry (1/500).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store lyophilized at 2-8 °C. After reconstitution aliquot and store at -20 °C.

Details zu Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Andere Bezeichnung: CD62E / E-Selectin (SELE Produkte)

Hintergrund: Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.Synonyms: CD62 antigen-like family member E, ELAM1, Endothelial leukocyte adhesion molecule 1, LECAM2, Leukocyte-endothelial cell adhesion molecule 2, SELE

Gen-ID: 20339

NCBI Accession: NP_035475

UniProt: Q00690

Pathways: Thromboxane A2 Receptor Signaling