Selectin E/CD62e Antikörper
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- Target Alle Selectin E/CD62e (SELE) Antikörper anzeigen
- Selectin E/CD62e (SELE) (Selectin E (SELE))
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Reaktivität
- Human, Maus
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Wirt
- Ratte
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Klonalität
- Monoklonal
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Konjugat
- Dieser Selectin E/CD62e Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)
- Kreuzreaktivität (Details)
- Species reactivity (tested):Human, Mouse.
- Aufreinigung
- Purified
- Immunogen
- LPS-activated mlEND1 cells expressing E-selectin at their cell surface
- Klon
- UZ4
- Isotyp
- IgM
- Top Product
- Discover our top product SELE Primärantikörper
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- Applikationshinweise
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UZ4 is a functional antibody in that it inhibits leukocyte adhesion to both murine andhuman activated endothelial cells. For in vivo application in mice 20 g of purified Ig perinjection is recommended. UZ4 is also suitable for immunoblotting, ELISA, immunoprecipitation and flow cytometry(dilution 1: 500). It is not optimal for immunohistochemistry, to analyze mouse E-selectin protein in frozensections, please refer to the anti E-selectin monoclonal antibodies UZ5 (ABIN112380), UZ6(ABIN112381), and UZ7 (ABIN112382).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- PBS, 0.09 % Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- Selectin E/CD62e (SELE) (Selectin E (SELE))
- Andere Bezeichnung
- CD62E / E-Selectin (SELE Produkte)
- Synonyme
- SELE antikoerper, CD62E antikoerper, ELAM antikoerper, ELAM1 antikoerper, ESEL antikoerper, LECAM2 antikoerper, E-selectin antikoerper, Elam antikoerper, selectin E antikoerper, selectin, endothelial cell antikoerper, SELE antikoerper, Sele antikoerper
- Hintergrund
- Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNF-alpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.Synonyms: CD62 antigen-like family member E, ELAM1, Endothelial leukocyte adhesion molecule 1, LECAM2, Leukocyte-endothelial cell adhesion molecule 2, SELE
- Gen-ID
- 9606
- UniProt
- P16581
- Pathways
- Thromboxane A2 Receptor Signaling
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