NEFH Antikörper
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- Target Alle NEFH Antikörper anzeigen
- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
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Reaktivität
- Human, Ratte, Maus, Huhn, Rind (Kuh), Meerschweinchen, Kaninchen, Hund, Schaf, Xenopus laevis
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser NEFH Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Spezifität
- RNF402 reacts with both the phosphorylated and non-phosphorylated isoforms of the 200 kDa Neurofilament protein. Reacts with Human, Rat, Rabbit, Dog, Hamster, Chicken, Mouse, Cow, Monkey, Sheep, Guinea Pig and Xenopus.
- Aufreinigung
- Purified
- Immunogen
- Neurofilament preparation of calf brain tissue.
- Klon
- RNF402
- Isotyp
- IgM
- Top Product
- Discover our top product NEFH Primärantikörper
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- Applikationshinweise
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ImmunoblottingImmunohistochemistry on Frozen and Paraffin Embedded Tissues. Recommended Dilutions: 1/10-1/100 for Immunohistochemistry with avidin biotinylatedHorseradish Peroxidase complex (ABC) as detection reagent, and 1/25-1/250 forImmunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- PBS, 0.09 % Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store lyophilized at 2 - 8 °C and reconstituted at -20 °C.
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- Target
- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
- Andere Bezeichnung
- Neurofilament H (200 kD) (NEFH Produkte)
- Synonyme
- NF-H antikoerper, NF200 antikoerper, Nfh antikoerper, mKIAA0845 antikoerper, NFH antikoerper, NEFH antikoerper, neurofilament heavy polypeptide antikoerper, neurofilament, heavy polypeptide antikoerper, neurofilament heavy antikoerper, MCYG_06231 antikoerper, Nefh antikoerper, NEFH antikoerper
- Hintergrund
- Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.Synonyms: (Neuronal Marker), 200 kDa neurofilament protein, KIAA0845, NEFH, NF-H, NFH, Neurofilament triplet H protein, heavy polypeptide
- Gen-ID
- 4744
- NCBI Accession
- NP_066554
- UniProt
- P12036
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