NEFH Antikörper (phosphospecific)

Produktnummer ABIN112359

Dieses Anti-NEFH-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von NEFH in WB, IHC (p) und IHC (fro). Geeignet für Human, Ratte, Maus, Huhn, Rind (Kuh), Kaninchen, Meerschweinchen, Hund, Schaf und Xenopus laevis.

Kurzübersicht für NEFH Antikörper (phosphospecific) (ABIN112359)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reaktivität: Human, Ratte, Maus, Huhn, Rind (Kuh), Kaninchen, Meerschweinchen, Hund, Schaf, Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NEFH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: RNF404

Produktdetails anti-NEFH Antikörper

Bindungsspezifität: phosphospecific

Spezifität: RNF404 reacts exclusively with the phosphorylated isoform of the 200 kDa neurofilament protein. Reacts with Bovine, Canine, Chicken, Guinea Pig, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xenopus.

Aufreinigung: Purified

Immunogen: Neurofilament preparation of calf brain tissue.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: RNF404 is suitable for Immunoblotting and Immunohistochemistry on frozen tissues. Recommended dilutions: 1/50-1/100 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/500 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store lyophilized at 2 - 8 °C and reconstituted at -20 °C.

Details zu NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Andere Bezeichnung: Neurofilament H (200 kD)

Hintergrund: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.Synonyms: (Neuronal Marker), 200 kDa neurofilament protein, KIAA0845, NEFH, NF-H, NFH, Neurofilament triplet H protein, heavy polypeptide

Gen-ID: 4744

NCBI Accession: NP_066554

UniProt: P12036