NEFM Antikörper (phosphospecific)

Produktnummer ABIN112358

Der Maus Monoklonal anti-NEFM Antikörper wird verwendet zum Nachweis von NEFM in Proben von Human, Kaninchen, Affe, Hamster, Xenopus laevis und Meerschweinchen. Er wurde validiert für WB, IHC (p) und IHC (fro).

Kurzübersicht für NEFM Antikörper (phosphospecific) (ABIN112358)

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Reaktivität: Human, Kaninchen, Affe, Hamster, Xenopus laevis, Meerschweinchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NEFM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: RNF406

Produktdetails anti-NEFM Antikörper

Bindungsspezifität: phosphospecific

Spezifität: RNF406 reacts exclusively with the phosphorylated isoform of the 160 kDa neurofilament protein. Reacts with Human, Rabbit, Hamster, Monkey, Guinea Pig and Xenopus.

Aufreinigung: Purified

Immunogen: Neurofilament preparation of calf brain tissue.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: RNF406 is suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry onfrozen sections and paraffin-embedded tissues and Flow cytometry. Recommended dilutions: 1/50-1/100 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/500 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store lyophilized at 2 - 8 °C and reconstituted at -20 °C.

Details zu NEFM

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Andere Bezeichnung: Neurofilament M (160 kD)

Hintergrund: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.Synonyms: (Neuronal Marker), NEF3, NEFM, NF-M, Neurofilament 3, Neurofilament medium polypeptide

Gen-ID: 4741

NCBI Accession: NP_005373

UniProt: P07197

Pathways: Brown Fat Cell Differentiation