Vimentin Antikörper

Produktnummer ABIN112354

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Vimentin in WB, IF, FACS, IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Schwein, Huhn, Affe, Hund, Ziege, Hamster und Zebrafisch (Danio rerio).

Kurzübersicht für Vimentin Antikörper (ABIN112354)

Target: Vimentin (VIM)

Reaktivität: Human, Maus, Ratte, Schwein, Huhn, Affe, Hund, Ziege, Hamster, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: RV202

Produktdetails anti-Vimentin Antikörper

Spezifität: RV202 reacts exclusively with Vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.

Kreuzreaktivität (Details): Species reactivity (tested):Canine, Chicken, Goat, Hamster, Human, Monkey, Mouse, Rat, Swine, Zebrafish.

Aufreinigung: Purified

Immunogen: Cytoskeletal vimentin extract of calf lens.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: RV202 is suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry onfrozen tissues and Flow cytometry. Recommended Dilutions: 1/100-1/200 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/1000 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the lyophilized antibody at 2-8 °C for one month or at -20 °C for longer. Aliquot and store the reconstituted antibody at -20 °C.

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: Vimentin

Hintergrund: Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.Synonyms: VIM

Gen-ID: 7431

NCBI Accession: NP_003371

UniProt: P08670

Pathways: Caspase Kaskade in der Apoptose