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Vimentin Antikörper

VIM Reaktivität: Human, Maus, Ratte, Schwein, Huhn, Affe, Hund, Ziege, Hamster, Zebrafisch (Danio rerio) WB, IF, FACS, IHC (p), IHC (fro) Wirt: Maus Monoclonal RV202 unconjugated

Produktnummer ABIN112354

Datenblatt (PDF)

Target Alle Vimentin (VIM) Antikörper anzeigen

Vimentin (VIM)
Reaktivität
320

129

125

61

59

49

42

37

30

21

20

13

13

8

7

6

6

3

3

2

1

1

1

1

1

1

1

1

1
Human, Maus, Ratte, Schwein, Huhn, Affe, Hund, Ziege, Hamster, Zebrafisch (Danio rerio)
Wirt
169

132

11

6

6

5

2

2
Maus
Passende Sekundärantikörper
Klonalität
183

149
Monoklonal
Konjugat
207

15

13

12

9

7

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

1

1
Dieser Vimentin Antikörper ist unkonjugiert
Applikation
271

117

111

96

92

63

47

40

39

32

23

23

8

6

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität

RV202 reacts exclusively with Vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.

Kreuzreaktivität (Details)

Species reactivity (tested):Canine, Chicken, Goat, Hamster, Human, Monkey, Mouse, Rat, Swine, Zebrafish.

Aufreinigung

Purified

Immunogen

Cytoskeletal vimentin extract of calf lens.

Klon

RV202

Isotyp

IgG1

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VIM Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Affe WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reaktivität: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM Reaktivität: Human, Maus, Ratte WB, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Säugetier WB, FACS, IHC (p), ICC Wirt: Maus Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human, Maus, Ratte, Schwein, Huhn WB, IHC (p), ICC, IP Wirt: Maus Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human, Maus WB, IF, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human WB, IHC, ICC, IP Wirt: Maus Monoclonal C8 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reaktivität: Human WB, IF, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) (AA 41-140) antibody
VIM Reaktivität: Human WB, ELISA, IF, IP Wirt: Maus Monoclonal 3E9 unconjugated

Applikationshinweise

RV202 is suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry onfrozen tissues and Flow cytometry. Recommended Dilutions: 1/100-1/200 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/1000 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

1.0 mg/mL

Buffer

PBS, 0.09 % Sodium Azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Avoid repeated freezing and thawing.

Lagerung

4 °C/-20 °C

Informationen zur Lagerung

Store the lyophilized antibody at 2-8 °C for one month or at -20 °C for longer. Aliquot and store the reconstituted antibody at -20 °C.
Target

Vimentin (VIM)

Andere Bezeichnung

Vimentin (VIM Produkte)

Hintergrund

Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.Synonyms: VIM

Gen-ID

7431

NCBI Accession

NP_003371

UniProt

P08670

Pathways

Caspase Kaskade in der Apoptose