Cytokeratin 13 Antikörper

Produktnummer ABIN112324

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Cytokeratin 13 in WB, IHC (p), IF und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Ratte und Rind (Kuh).

Kurzübersicht für Cytokeratin 13 Antikörper (ABIN112324)

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Reaktivität: Human, Ratte, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 13 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: Ks13-1

Produktdetails anti-Cytokeratin 13 Antikörper

Spezifität: Ks 13.1 represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin. Polypeptide reacting: Mr 54 000 polypeptide human keratin K13 (formerly designated cytokeratin13, with minor affinity to keratins K14, Mr 50 000, and K16, Mr 48 000). Tumors specifically detected: several squamous cell carcinomas, e.g. cervix carcinoma, transitional cell carcinoma of the bladder, craniopharyngioma.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Bovine and Rat.

Aufreinigung: Protein A affinity chromatography

Immunogen: Keratin K13 of Mr 54.000 purified from human esophagus.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunofluorescence. Immunohistochemistry on frozen and paraffin sections: 1: 10, protease pretreatmentimproves the performance of this excellent marker in immunohisto-chemistry: 1/10 withPBS, pH 7.4. Western Blot. Incubation time: 1h at RT, extended with parafin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in 1 mL dist. water.

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Cytokeratin 13

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Andere Bezeichnung: Cytokeratin 13

Hintergrund: Cytokeratin 13 is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus.Synonyms: CK-13, CK13, K13, KRT-13, KRT13, Keratin type I cytoskeletal 13, Keratin-13

Gen-ID: 3860

NCBI Accession: NP_002265

UniProt: P13646