KRT4 Antikörper

Produktnummer ABIN112320

Zitiert in 1 Publikation. Der Maus Monoklonal anti-KRT4 Antikörper (Klon 6B10) (ABIN112320) detektiert spezifisch KRT4 in WB, IF, FACS, IHC (p) und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Hund, Cat und Zebrafisch (Danio rerio).

Kurzübersicht für KRT4 Antikörper (ABIN112320)

Target: KRT4 (Keratin 4 (KRT4))

Reaktivität: Human, Hund, Cat, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KRT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 6B10

Produktdetails anti-KRT4 Antikörper

Spezifität: This Monoclonal antibody 6B10 reacts exlcusively with Cytokeratin 4 which is present in non-cornifying squamous epithelium, including cornea and transitional epithelium. Cells in certain ciliated pseudo-stratified epithelia and ductal epithelia of various exocrine glands are also positive for 6B10.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Canine, Feline, Zebrafish.

Aufreinigung: Purified

Immunogen: 6B10 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytokeratin preparation extracted from human esophagus.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry. Immunohistochemistry on Frozen and Tissues (1/25-1/200) using with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent. Immunoblotting (1/100-1/1000). Flow Cytometry (1/25-1/200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-KRT4 Antikörper (ABIN112320)

Liu, Li, Tan, Beuerman: "The eyelid margin: a transitional zone for 2 epithelial phenotypes." in: Archives of ophthalmology (Chicago, Ill. : 1960), Vol. 125, Issue 4, pp. 523-32, (2007) (PubMed).

Details zu KRT4

Target: KRT4 (Keratin 4 (KRT4))

Andere Bezeichnung: Cytokeratin 4

Hintergrund: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK4, CYK4, Cytokeratin-4, K4, KRT4, Keratin, Keratin-4, type II cytoskeletal 4

Gen-ID: 9606

UniProt: P19013