Uroplakin 3A Antikörper

Produktnummer ABIN112250

Der Maus Monoklonal Anti-Uroplakin 3A-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, Uroplakin 3A in Proben von Human, Ratte, Rind (Kuh) und Schwein zu detektieren.

Kurzübersicht für Uroplakin 3A Antikörper (ABIN112250)

Target: Uroplakin 3A (UPK3A)

Reaktivität: Human, Ratte, Rind (Kuh), Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Uroplakin 3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: AU1

Produktdetails anti-Uroplakin 3A Antikörper

Spezifität: Clone AU1 reacts specifically with Uroplakin III present in the superficial cell layer of the urothelium. The binding region has been localized on the extracellular part of the antigen. Antibody AU1 strongly stains the urothelial surface membrane in paraffin sections of human renal pelvis, ureter, bladder, and urethra. About 60 % of human transitional cell carcinomas (including metastases) maintain focal (sometimes very limited) expression of Uroplakin III. Until now, no Uroplakin staining was found in any non-urothelial carcinoma (Moll et al., 1995). Uroplakin III may thus serve as a specific urothelial differentiation marker in cases of metastatic carcinomas with unclear primary tumor.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Bovine, Pig and Rat.

Aufreinigung: Affinity Chromatography on Protein A.

Immunogen: Asymmetric unit membrane (AUM) preparation from bovine urinary bladder.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoblotting (Western blotting). Immunohistochemistry on Paraffin-Embedded Tissue (not on Frozen Sections! ) using aheat-induced retrieval method (e. g. microwave treatment): Working dilution: 1/10. Incubation Time: 1h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1.0 mL distilled water.

Buffer: Final solution contains PBS, pH 7.4 with 0.5 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu Uroplakin 3A

Target: Uroplakin 3A (UPK3A)

Andere Bezeichnung: Uroplakin-3a (UPK3A)

Hintergrund: Together with the Uroplakins UP Ia, UP Ib and UP II, Uroplakin III contributes in constituting the asymmetrical unit membrane of the plaques of urothelial superficial (umbrella) cells. Uroplakin is a membrane glycoprotein (47 kD) and has been shown to be a specific marker of terminal urothelial differentiation (Wu et al., 1993, 1994).Synonyms: UP3a, UPIII, UPK3, Uroplakin III

Gen-ID: 7380

NCBI Accession: NP_001161046

UniProt: O75631