JUP Antikörper

Produktnummer ABIN112236

Dieses Anti-JUP-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von JUP in WB, IF, IHC (p), IP und IHC (fro). Geeignet für Human, Maus, Ratte, Huhn, Rind (Kuh) und Zebrafisch (Danio rerio).

Kurzübersicht für JUP Antikörper (ABIN112236)

Target: JUP (Junction Plakoglobin (JUP))

Reaktivität: Human, Maus, Ratte, Huhn, Rind (Kuh), Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser JUP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: PG 5-1

Produktdetails anti-JUP Antikörper

Spezifität: PG 5.1 represents an excellent marker for all forms of intercellular adhering junctions, such as: Desmosomes of epithelial and myocardial cells (incl. cultured cells), Zonulae and fasciae adherentes of epithelia, endothelia of blood vessels and myocardial cells, adherens-type junctions (e.g. lens tissue, pigmented retinal cells, Sertoli cells of testis). The PG 5.1 epitope maps within the C-terminus at the extreme end of repeat 13 (aa 632-687) of plakoglobin. Polypeptide Reacting: Mr 83 000 Plakoglobin, "band 5" polypeptide of intercellular adhering junctions (identical to γ-catenin). Reactivity on Cultured Cell Lines: Cell cultures forming monolayers (from tested species cited below).

Kreuzreaktivität (Details): Species reactivity (tested):Human, Bovine, Rat, Mouse, Chicken and Zebrafish.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Plakoglobin, "band 5" protein from Bovine snout epidermis

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunoblotting. Immunoprecipitation. Immunofluorescence Microscopy: 1/5 (1 h at RT). Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in 1 mL distilled water

Buffer: Final solution contains PBS buffer, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu JUP

Target: JUP (Junction Plakoglobin (JUP))

Andere Bezeichnung: Junction Plakoglobin

Hintergrund: Gamma Catenin is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of gamma Catenin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. It is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat.Synonyms: CTNNG, Catenin gamma, DP3, Desmoplakin III, Desmoplakin-3, JUP

Gen-ID: 3728

NCBI Accession: NP_002221

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location