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SYNPO Antikörper

SYNPO Reaktivität: Human, Maus, Ratte, Rind (Kuh), Wüstenrennmaus, Meerschweinchen WB, IF, IHC (p), IHC (fro) Wirt: Maus Monoclonal G1D4 unconjugated
Produktnummer ABIN112223
  • Target Alle SYNPO Antikörper anzeigen
    SYNPO (Synaptopodin (SYNPO))
    Reaktivität
    • 34
    • 28
    • 25
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Rind (Kuh), Wüstenrennmaus, Meerschweinchen
    Wirt
    • 48
    • 3
    • 3
    Maus
    Klonalität
    • 51
    • 3
    Monoklonal
    Konjugat
    • 18
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser SYNPO Antikörper ist unkonjugiert
    Applikation
    • 42
    • 14
    • 13
    • 10
    • 6
    • 6
    • 5
    • 5
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Spezifität
    The antibody reacts specifically with Synaptopodin. It recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-Actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation dependent expression during postnatal maturation of Rat brain. Differentiation dependent expression in cultured hippocampal neurons.
    Kreuzreaktivität (Details)
    Species reactivity (tested):Human, Bovine, Rat, Mouse, Guinea Pig and Gerbil. Negative with Rabbit, Frog and Chicken.
    Aufreinigung
    Affinity chromatography on Protein A
    Immunogen
    Isolated Rat kidney glomeruli.
    Klon
    G1D4
    Isotyp
    IgG1
    Top Product
    Discover our top product SYNPO Primärantikörper
  • Applikationshinweise
    Immunofluorescence. Immunohistochemistry on Frozen SectionsImmunohistochemistry on Paraffin Embedded Tissue: 1/5-1/10 for 1 h at RT (Aftermicrowave treatment). Immunoblotting (Western): 0.1-0.5 μg/mL. The antibody also reacts with a 44 kD degradation fragment of Synaptopodin.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Rekonstitution
    Restore with 1 mL distilled water
    Buffer
    Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
  • Tan, Rak-Raszewska, Skovorodkin, Vainio: "Mouse Embryonic Stem Cell-Derived Ureteric Bud Progenitors Induce Nephrogenesis." in: Cells, Vol. 9, Issue 2, (2021) (PubMed).

    Xu, Chen, Dong, Meyuhas, Chen: "Phosphorylation of ribosomal protein S6 mediates compensatory renal hypertrophy." in: Kidney international, Vol. 87, Issue 3, pp. 543-56, (2015) (PubMed).

    Yates, Papakrivopoulou, Long, Goggolidou, Connolly, Woolf, Dean: "The planar cell polarity gene Vangl2 is required for mammalian kidney-branching morphogenesis and glomerular maturation." in: Human molecular genetics, Vol. 19, Issue 23, pp. 4663-76, (2010) (PubMed).

    Câmpean, Karpe, Haas, Atalla, Peters, Rupprecht, Liebner, Acker, Plate, Amann: "Angiopoietin 1 and 2 gene and protein expression is differentially regulated in acute anti-Thy1.1 glomerulonephritis." in: American journal of physiology. Renal physiology, Vol. 294, Issue 5, pp. F1174-84, (2008) (PubMed).

    Lechler, Wu, Bernhardt, Campean, Gastiger, Hackenbeck, Klanke, Weidemann, Warnecke, Amann, Engehausen, Willam, Eckardt, Rödel, Wiesener: "The tumor gene survivin is highly expressed in adult renal tubular cells: implications for a pathophysiological role in the kidney." in: The American journal of pathology, Vol. 171, Issue 5, pp. 1483-98, (2007) (PubMed).

  • Target
    SYNPO (Synaptopodin (SYNPO))
    Andere Bezeichnung
    Synaptopodin / SYNPO (SYNPO Produkte)
    Synonyme
    SYNPO antikoerper, DKFZp459N012 antikoerper, 9030217H17Rik antikoerper, 9130229N11 antikoerper, 9330140I15Rik antikoerper, AW046661 antikoerper, synaptopodin antikoerper, formin-like protein 5 antikoerper, SYNPO antikoerper, LOC100147046 antikoerper, Synpo antikoerper
    Hintergrund
    Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data), in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).Synonyms: KIAA1029
    Gen-ID
    11346, 9606
    UniProt
    Q8N3V7
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