Vimentin Antikörper

Produktnummer ABIN112184

Der Maus Monoklonal Anti-Vimentin Antikörper wurde für WB, IF, FACS, IHC (p) und IHC (fro) validiert. Er ist geeignet, Vimentin in Proben von Human, Ratte, Huhn, Schaf und Potoroo zu detektieren.

Kurzübersicht für Vimentin Antikörper (ABIN112184)

Target: Vimentin (VIM)

Reaktivität: Human, Ratte, Huhn, Schaf, Potoroo

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: V9

Produktdetails anti-Vimentin Antikörper

Spezifität: The antibody clone V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.

Aufreinigung: Purified

Immunogen: V9 is an antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with Vimentin isolated from Porcine lens.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: This antibody is suitable for Immunoblotting, Immunocytochemistry,Immunohistochemistry on frozen sections and paraffin-embedded tissues and Flowcytometry. Recommended dilutions: Flow cytometry: 1/25-1/200. Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) asdetection reagent: 1/25-1/200. For staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer ( pH 6.0) is required. Immunoblotting applications: 1/100-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Do not freeze working dilutions.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the original vial at 2-8 °C. After reconstitution, aliquot and store stock solution or aliquots thereof at -20 °C.

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: Vimentin

Hintergrund: Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP, often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.Synonyms: VIM

Gen-ID: 7431

NCBI Accession: NP_003371

UniProt: P08670

Pathways: Caspase Kaskade in der Apoptose