KRT8 Antikörper

Produktnummer ABIN112171

Produktdetails anti-KRT8 Antikörper

Target: KRT8 (Keratin 8 (KRT8))

Reaktivität: Human, Ratte, Maus, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KRT8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Ks 8.7 represents an excellent marker to discriminate simple epithelia from those of different origin. Polypetide Reacting: Mr 52500 polypeptide (keratin K8, formerly also designated cytokeratin 8) of human epithelia. Tumors Specifically Detected: All adeno-CA tested, undifferentiated CA, cervix CA, hepatocellular CA. Tested Reactivities on cultured cell lines: MCF-7, RT 112, PLC.

Aufreinigung: Protein A affinity chromatography

Immunogen: Cytoskeletal proteins from cultured HeLa cells

Klon: Ks8-7

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot. Immunocytology. Immunohistochemistry (1: 10) on frozen tissues and paraffin-embedded sections aftermicrowave treatment. Incubation time: 1 h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in 1 mL distilled water.

Buffer: Final solution contains 0.09 % Sodium Azide and 0.5 % BSA in PBS buffer, pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu KRT8

Target: KRT8 (Keratin 8 (KRT8))

Andere Bezeichnung: Cytokeratin 8 (KRT8 Produkte)

Hintergrund: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual cytokeratin polypeptides are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.Synonyms: CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, type II cytoskeletal 8

Gen-ID: 3856

NCBI Accession: NP_002264

UniProt: P05787