Cytokeratin 5 Antikörper (Biotin)

Produktnummer ABIN112168

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Cytokeratin 5 in IF, FACS, IHC (p), IHC (fro) und EIA. Er zeigt eine Reaktivität gegenüber Human, Maus, Rind (Kuh), Schwein, Hydra und Amphibien.

Kurzübersicht für Cytokeratin 5 Antikörper (Biotin) (ABIN112168)

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Reaktivität: Human, Maus, Rind (Kuh), Schwein, Hydra, Amphibien

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 5 Antikörper ist konjugiert mit Biotin

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: C22

Produktdetails anti-Cytokeratin 5 Antikörper (Biotin)

Spezifität: Antibody clone C22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with Keratins K5 and K8 present in nearly all epithelia. Polypeptide Reacting: Mr 52 500, Mr 58 000 keratins (type II keratins K5 and K8, formerly also designated cytokeratins 5 and 8) of human epithelial cells. Reactivities on Cultured Cell Lines (tested so far): MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK. Epitope has been mapped to aa 353-367 on alpha helical rod domain (See Waseem et al., 2004).

Kreuzreaktivität (Details): Also reacts to: KRT8

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Human keratin K8, purified from SDS PAGE gel

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Flow Cytometry. Immunocytology. Immunohistochemistry on Frozen Tissues (1/10-1/20), Incubation time: 1 h at RT. Immunohistochemistry on Paraffin-Embedded Tissues (1/10-1/20), Incubation time: overnight at 2-8 °C. With paraffin-embedded sections, protease pretreatment is requiredprior to antibody application.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Andere Bezeichnung: Cytokeratin 5

Hintergrund: Cytokeratin 5 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.Synonyms: 58 kDa Cytokeratin, CK5, Cytokeratin-5, K5, KRT5, Keratin 5, Keratin type II cytoskeletal 5, Keratin-5

Gen-ID: 3852

NCBI Accession: NP_000415

UniProt: P13647