Desmoglein 2 Antikörper
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- Target Alle Desmoglein 2 (DSG2) Antikörper anzeigen
- Desmoglein 2 (DSG2)
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Desmoglein 2 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- Spezifität
- The antibody recognizes Desmoglein 2 present in low concentrations in all epithelia and in tumors derived therefrom as well as in heart muscle and dendritic reticulum cells of lymphatic follicles. In basal epithelial cells desmoglein 2 expression is generally very low. Polypeptide Reacting: Desmoglein 2 (1069aa, calculated Mol. Wt. 116,760). Reactivity on Cultured Cell Lines: Many, including CaCo-2, HaCaT, MCF-7. Negative with Mouse and Rat.
- Aufreinigung
- Affinity Chromatography on Protein A
- Immunogen
- Recombinant peptide (220aa of 2nd and 3rd extracellular repeat domain)
- Klon
- 10G11
- Isotyp
- IgG1
- Top Product
- Discover our top product DSG2 Primärantikörper
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- Applikationshinweise
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ELISA. Western blot. Immunofluorescence. Immunohistochemistry on Frozen Sections: For better resolution preincubation (directlyafter fixation) with 0.05-0.2 % Triton X-100 for 5-10 min, depending on tissue type, isrecommended (see Ref.3). Immunohistochemistry on Paraffin Sections: Use microwave treatment. Incubation Time: 1 h at RT for Frozen Tissue and overnight at 2-8 °C for Paraffin Sections. Working Dilution: Dilute 1/10 with PBS, pH 7.4 for Immunohistochemical applications . Recommended Positive Control: CaCo-2, HaCaT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Rekonstitution
- Restore in 1 mL distilled water
- Buffer
- Final solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
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Desmoglein 2-mediated adhesion is required for intestinal epithelial barrier integrity." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 298, Issue 5, pp. G774-83, (2010) (PubMed).
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Desmoglein 2-mediated adhesion is required for intestinal epithelial barrier integrity." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 298, Issue 5, pp. G774-83, (2010) (PubMed).
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- Target
- Desmoglein 2 (DSG2)
- Andere Bezeichnung
- Desmoglein-2 (DSG2 Produkte)
- Synonyme
- DSG2 antikoerper, dsc antikoerper, dsga antikoerper, si:dkeyp-51f12.6 antikoerper, wu:fj23f02 antikoerper, ARVC10 antikoerper, ARVD10 antikoerper, CDHF5 antikoerper, CMD1BB antikoerper, HDGC antikoerper, AA408168 antikoerper, D18Ertd293e antikoerper, desmoglein 2 antikoerper, desmoglein 2, tandem duplicate 1 antikoerper, DSG2 antikoerper, dsg2.1 antikoerper, dsg2 antikoerper, Dsg2 antikoerper
- Hintergrund
- Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 2 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines.Synonyms: CDHF5, Cadherin family member 5, DSG2, HDGC
- Gen-ID
- 1829
- NCBI Accession
- NP_001934
- UniProt
- Q14126
- Pathways
- Cell-Cell Junction Organization
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