BMP6 Antikörper (N-Term) (Biotin)

Produktnummer ABIN111999

Dieses Anti-BMP6-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von BMP6 in IHC (p). Geeignet für Human und Ratte.

Kurzübersicht für BMP6 Antikörper (N-Term) (Biotin) (ABIN111999)

Target: BMP6 (Bone Morphogenetic Protein 6 (BMP6))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BMP6 Antikörper ist konjugiert mit Biotin

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: Morph-6-1

Produktdetails anti-BMP6 Antikörper (Biotin)

Bindungsspezifität: AA 1-29, N-Term

Spezifität: This antibody reacts with Bone morphogenic Protein-6 (BMP-6). Antigen specificity of morph-6.1: Pre-incubation of morph-6.1 with amino-terminal peptide 1-29 of BMP-6 inhibits binding of the mAb to tissue sections. Pre-incubation with other amino-terminal synthetic peptides of human BMP-7, BMP-4, BMP-3 and BMP-2 as well as an irrelevant peptide does not inhibit specific tissue staining. Antigen Distribution on Tissue Sections: Positive staining can be observed in embryonic and adult central nervous system. Rat radial glial cells of the developing central nervous system from E11 to E19. In rat peripheral nerves a selective intracellular immunoreactivity can be found in perinuclear region of most Schwann cells which form the myelin sheath. However, some Schwann cells were negative for morph-6.1. BMP-6 can also be found in a variety of other tissues and cell types, notably keratinizing epithelial cells. Smooth muscle cells, characterized by Desmin positivity, were positively stained in normal tissues and in atherosclerotic plaques. Macrophages (CD68+) and endothelial cells were negative with morph-6.1.

Kreuzreaktivität (Details): Species reactivity (tested):Rat, Human (Phylogenetically highly conserved antigen).

Aufreinigung: Affinity Chromatography.

Immunogen: Aminoterminal synthetic peptide 1-29.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen Sections: 1-2 μg/mL (1/100-1/200). Immunohistochemistry on Paraffin Sections (Microwave pretreatment for antigen retrievalis recommended). Recommended Dilutions: Rat Brain.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 with 0.09 % Sodium Azide as preservative and 5 mg/mL BSA as stabilizer

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Details zu BMP6

Target: BMP6 (Bone Morphogenetic Protein 6 (BMP6))

Andere Bezeichnung: BMP6

Hintergrund: Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species. BMPs are involved in embryogenesis and morphogenesis of various tissues and organs. BMP6 is an autocrine stimulator of chondrocyte differentiation and has been implicated in the development of embryonic kidney and urinary systems. It is involved in liver growth and differentiation, keratinocyte differentiation, and regulation of neuronal tissue development. BMP6 expression is localized to smooth muscle cells of normal cerebral blood vessels, intimal plaques and atherosclerotically changed blood vessels, further suggesting a role as a regulator of developing tissues. BMP-6 is a member of the TGF-beta superfamily of cytokines regulating homeotic gene expression, embryonic development and neurogenesis.Synonyms: BMP-6, Bone morphogenetic protein 6, VGR, VGR1

Gen-ID: 25644

NCBI Accession: NP_037239

UniProt: Q04906

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process