CD4 Antikörper
-
- Target Alle CD4 Antikörper anzeigen
- CD4
-
Reaktivität
- Ratte
-
Wirt
- Maus
-
Klonalität
- Monoklonal
-
Konjugat
- Dieser CD4 Antikörper ist unkonjugiert
-
Applikation
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
- Aufreinigung
- Affinity chromatography.
- Immunogen
- Lymphoblasts.
- Klon
- Rib 5-2
- Isotyp
- IgG2a
- Top Product
- Discover our top product CD4 Primärantikörper
-
anti-CD4 (CD4) (AA 385-457) antibody
CD4 Reaktivität: Human, Maus, Ratte, Huhn, Schwein FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Wirt: Kaninchen Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 Reaktivität: Human, Maus, Ratte, Huhn, Affe, Hund, Schwein, Cat, Rind (Kuh), Kaninchen, Schaf, Meerschweinchen, Ziege, Pferd, Avian, Esel, Hamster WB Wirt: Ziege Polyclonal unconjugated
anti-CD4 (CD4) (AA 231-330) antibodyCD4 Reaktivität: Human, Maus, Ratte FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Wirt: Kaninchen Polyclonal unconjugated
anti-CD4 (CD4) (N-Term) antibodyCD4 Reaktivität: Human FACS, WB, CyTOF Wirt: Maus Monoclonal MEM-241 unconjugated
anti-CD4 (CD4) antibodyCD4 Reaktivität: Maus FACS, IP, IHC (fro), ICC, Func Wirt: Ratte Monoclonal GK1-5 unconjugated
anti-CD4 (CD4) (N-Term) antibody (FITC)CD4 Reaktivität: Human FACS Wirt: Maus Monoclonal MEM-241 FITC
anti-CD4 (CD4) (AA 301-400) antibodyCD4 Reaktivität: Human, Maus, Ratte FACS, WB, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
anti-CD4 (CD4) (N-Term) antibody (PE)CD4 Reaktivität: Human FACS Wirt: Maus Monoclonal MEM-241 PE
anti-CD4 (CD4) antibody (PE)CD4 Reaktivität: Ratte FACS Wirt: Maus Monoclonal OX-35 PE
anti-CD4 (CD4) (AA 26-200) antibodyCD4 Reaktivität: Human WB, IF Wirt: Kaninchen Polyclonal unconjugated
-
- Applikationshinweise
-
Suitable for Immunohistochemistry (IHC), has been described to work in FACS (see Table1). Recommended Dilutions for IHC: Frozen sections: 1.5 μg/mL (1/200)Paraffin sections: does not react on routinely processed paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protokoll
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L)minimal-cross reaction to rat) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Rekonstitution
- Restore with 0.5 mL distilled water.
- Konzentration
- 0.3 mg/mL
- Buffer
- PBS, pH 7.2 containing 0.01 % Thiomerosal as preservative and 10 mg/mL BSA as stabilizer.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody at 2-8 °C for one month or at -20 °C for longer.
-
- Target
- CD4
- Andere Bezeichnung
- CD4 (CD4 Produkte)
- Hintergrund
- CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
- Gen-ID
- 10116
- UniProt
- P05540
- Pathways
- T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events
-
