MHC Class II (I-A K,s,r) Antikörper

Produktnummer ABIN111990

Dieses Anti--Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von in FACS und IHC (fro). Geeignet für Maus.

Kurzübersicht für MHC Class II (I-A K,s,r) Antikörper (ABIN111990)

Target: MHC Class II (I-A K,s,r)

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: ER-TR2

Produktdetails

Aufreinigung: Affinity Chromatography

Immunogen: Murine thymic reticulum Remarks: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34kDa) and one beta-chain (26-29kDa). The epitope has not been further characterized.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on frozen sections (1 μg/mL, 1: 200). Suggested positive control: Mouse spleen. FACS. Does not react on routinely processed paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water (=stock solution).

Konzentration: 0.2 mg/mL

Buffer: Phosphate buffered saline, pH 7.2 (PBS), 0.01 % Thimerosal, 10 mg/mL BSA

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2 - 8 °C.

Antigendetails

Target: MHC Class II (I-A K,s,r)