MHC Class II Antigen I Ak,d,b,q,r Antikörper (Biotin)

Produktnummer ABIN111988

Produktdetails

Target: MHC Class II Antigen I Ak,d,b,q,r

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Keine Kreuzreaktivität: Human

Kreuzreaktivität (Details): Species reactivity (tested):Mouse (Cells expressing MHC class II antigens).

Aufreinigung: Affinity Chromatography

Immunogen: Murine thymic reticulum Remarks: Antigen / Epitope: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34 kDa) and one beta-chain (26-29 kDa). The epitope has not been further characterized.

Klon: ER-TR3

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen Sections: 4 μg/mL (1/100). Suggested Positive Control: Mouse spleen. Does not react on routinely processed paraffin sections. Has been reported to work in Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 0.4 mg/mL

Buffer: Stock Solution contains PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer with no preservative

Konservierungsmittel: Without preservative

Handhabung: This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Lagerung: 4 °C

Informationen zur Lagerung: Store at +4 °C. DO NOT FREEZE. This product should be stored undiluted.

Antigendetails

Target: MHC Class II Antigen I Ak,d,b,q,r

Andere Bezeichnung: MHC Class II (I-A K,b,d,q,r)

Hintergrund: MHC Class II antigens are heterodimers consisting of one alpha chain (31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR3, ER-TR2, ER-TR1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the Human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.