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Endothelial Cells Antikörper

Dieses Anti-Endothelial Cells-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von Endothelial Cells in IHC (fro). Geeignet für Human.
Produktnummer ABIN111945

Kurzübersicht für Endothelial Cells Antikörper (ABIN111945)

Target

Endothelial Cells

Reaktivität

  • 4
  • 3
  • 2
Human

Wirt

  • 6
  • 3
Ratte

Klonalität

  • 9
Monoklonal

Konjugat

  • 7
  • 2
Dieser Endothelial Cells Antikörper ist unkonjugiert

Applikation

  • 9
  • 3
  • 2
  • 1
  • 1
  • 1
Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon

1F10
  • Aufreinigung

    Affinity A chromatography.

    Immunogen

    Cultured HUVECs.

    Isotyp

    IgG2a
  • Applikationshinweise

    Immunohistochemistry on Frozen sections: 2 μg/mL (1: 100),Suggested positive control: Human placenta. Antigen Distribution: The antigen is found in variable amounts on freshly isolated isolated HUVE cells. Antigenexpression on cultured HUVEC is dependent on the culture conditions. Best results wereobtained (40-70 % pos. ) by daily renewal of FCS containing medium supplemented withendothelial cell conditioned supernatant whereas commercial available specificendothelial cell media have no influence on the antigen expression (see Table 1).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Rekonstitution

    Restore with 0.5 mL distilled water.

    Konzentration

    0.2 mg/mL

    Buffer

    PBS, pH 7.2 containing 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.01 % thimerosal as a preservative.

    Konservierungsmittel

    Thimerosal (Merthiolate)

    Vorsichtsmaßnahmen

    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Dilute only prior to immediate use

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
  • Target

    Endothelial Cells

    Hintergrund

    Synonyms: Endothelial Cell marker
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