CD9 Antikörper

Produktnummer ABIN111919

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD9 in FACS, WB, IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Hund, Kaninchen, Cat und Affe.

Kurzübersicht für CD9 Antikörper (ABIN111919)

Target: CD9

Reaktivität: Human, Hund, Kaninchen, Cat, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD9 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: K41

Produktdetails anti-CD9 Antikörper

Spezifität: Clone K41 detects CD9. Antigen distribution on Isolated cells: CD9 is mainly expressed on platelets, on early B-cells, and monocytes. Further reactivity could be seen with basophiles and eosinophiles. CD9 is used for the characterization of leukaemias and lymphomas. 90 % of non-T acute lymphoblastic leukaemic cells, 50 % of acute myeloid as well as chronic lymphoid leukaemias express the antigen. Tissue sections: glomeruli, sinus histiocytes, germinal centre, brain white matter, nerves, skin, and vascular smooth muscles.

Aufreinigung: Affinity chromatography.

Immunogen: Vero cells

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Suitable for Immunohistochemistry on: Frozen sections: 2-5 μg/mL, 1/80-1/200). Paraffin sections: 8 μg/mL, 1/50, pretreatment is not necessary. Suggested positive control: human tonsil. Also works in FACS and Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 0.4 mg/mL

Buffer: PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % thimerosal as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD9

Target: CD9

Andere Bezeichnung: CD9

Hintergrund: CD9 is a member of the tetraspan or transmembrane 4 superfamily. The family members (CD9, CD63, CD37, Cd53, CD81, CD82, CD151) show a high degree of homology in their membrane-spanning domain. CD9 is the only member with N-linked sugar bound to the first extracellular segment. Tetraspans are expressed by many cell types and show a different subcellular distribution. CD9 is constitutively surface-expressed by platelets and pre-B cells and is present in the alpha-granules of platelets. The participation of different tetraspan members in signalling complexes on B and T cells have been described. Furthermore, association of CD9 with beta1 integrins has been found. Anti CD9 monoclonal antibodies induce homotypic pre-B cell aggregation and fibronectin receptor-mediated adherence of these cells to bone marrow fibroblasts. Recent observations suggest a role of CD9 in adhesion.Synonyms: 5H9 antigen, Cell growth-inhibiting gene 2 protein, GIG2, Leukocyte antigen MIC3, MIC3, Motility-related protein, TSPAN29, Tetraspanin-29, p24

Gen-ID: 928

NCBI Accession: NP_001760

UniProt: P21926

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization