CD163 Antikörper (FITC)

Produktnummer ABIN111917

Für dieses Produkt sind 2+ Publikationen verfügbar. Der Maus-Monoklonal Anti-CD163-Antikörper wurde für FACS validiert. Er ist geeignet zum Nachweis von CD163 in Proben aus Human. }

Kurzübersicht für CD163 Antikörper (FITC) (ABIN111917)

Target: CD163

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD163 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Klon: 5C6-FAT

Produktdetails anti-CD163 Antikörper (FITC)

Spezifität: Monoclonal antibody 5C6 FAT recognizes a membrane Glycoprotein on Monocytes and Macrophages which is expressed in intermediate and late inflammatory stages. Antigen Distribution Isolated Cells: Monocytes, particularly after dexamethasone treatment or after 2-5 days in culture. Does not react with lymphocytes, granulocytes or platelets. Tissue Sections: Positive staining can be observed in the skin (histiocytes), gut, Kupffer cells, few alveolar macrophages, a major population of macrophages in the placenta, varying degrees of macrophages in inflamed tissues, including tumorous tissue depending on the inflammatory stage. Red pulp, but not white pulp macrophages of the spleen, and cortical macrophages of the thymus are detected. Macrophages in the synovialis of patients with rheumatoid arthritis. In alveolar macrophages and in Kupffer cells a double staining can be observed with monoclonal antibody 25F9 (product BM4022) which is not the case in other tissues. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions.

Kreuzreaktivität (Details): Species reactivity (tested):Human: Monocytes and Macrophages. Negative with Pig.

Aufreinigung: Affinity Chromatography

Immunogen: Human CD163. Remarks: The epitope has not been further characterized.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 50 μL of 1/10-1/20 diluted antibody to label 5x10e5 cells. Suggested Positive Control: Human monocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, 0.05 % Sodium Azide, 10 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-CD163 Antikörper (FITC) (ABIN111917)

Bir, Calli-Demirkan, Tufan, Akbulut, Satiroglu-Tufan: "Apoptotic cell death and its relationship to gastric carcinogenesis." in: World journal of gastroenterology : WJG, Vol. 13, Issue 23, pp. 3183-8, (2007) (PubMed).

Zaba, Cardinale, Gilleaudeau, Sullivan-Whalen, Suárez-Fariñas, Suárez Fariñas, Fuentes-Duculan, Novitskaya, Khatcherian, Bluth, Lowes, Krueger: "Amelioration of epidermal hyperplasia by TNF inhibition is associated with reduced Th17 responses." in: The Journal of experimental medicine, Vol. 204, Issue 13, pp. 3183-94, (2007) (PubMed).

Details zu CD163

Target: CD163

Andere Bezeichnung: CD163

Hintergrund: CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163-positive cells include skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and some thymus macrophages. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130

Gen-ID: 9332

NCBI Accession: NP_004235

UniProt: Q86VB7