CD93 Antikörper (Biotin)

Produktnummer ABIN111896

Produktdetails anti-CD93 Antikörper (Biotin)

Target: CD93

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD93 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This Monoclonal antibody clone X-2 recognizes a 110 kDa O-sialoglycoprotein on granulocytes, monocytes and endothelial cells. Upon differentiation of cultivated monocytes into macrophages, CDw93 ceases to be expressed on day 5-6, which makes it a very useful marker to differentiate those two cell populations. Antigen Distribution Isolated Cells: Positive with all granulocytes, with staining increasing transiently upon maturation. Antigen expression is increased by TNFα, GM-CSF, (fMLP) treatment. No effect on superoxide production by binding to neutrophils has been noted. PMA stimulation causes down regulation of the antigen. Positive with monocytes following fMLP, TNF, GM-CSF treatment. Antigen expression decreases on cultured monocytes. Positive on AML cases with monocytic component. Positive on cell lines HL-60, Namalva, K562, THP-1, U937, negative on HMC-1 (human mast cell line). Tissue Sections: The Monoclonal antibody X-2 stains epitheloid cells and Rosai-Dorfman cells and most endothelial cells in the synovial membrane of RA patients.

Aufreinigung: Affinity Chromatography

Immunogen: Human monocytes. Remarks: The antigen has been assigned to the CDw93 cluster, the epitope has not been further characterized.

Klon: X-2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Suitable for Immunohistochemistry (IHC) on: Frozen sections: 0.5 μg/mL (1/400)Paraffin sections: does not react on routinely processedparaffin sections. It works alos in FACS. Suggested positive control: human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water

Konzentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, 0.01 % Thimerosal, 10 mg/mL BSA

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C before reconstitution. After reconstitution store at 2-8° for up to 2 months. DO NOT FREEZE. Recontstituted product is stable for 2 days at RT.

Referenzen für anti-CD93 Antikörper (Biotin) (ABIN111896)

Sumide, Matsuoka, Kawamura, Nakatsuka, Fujioka, Asano, Takihara, Sonoda: "A revised road map for the commitment of human cord blood CD34-negative hematopoietic stem cells." in: Nature communications, Vol. 9, Issue 1, pp. 2202, (2018) (PubMed).

Details zu CD93

Target: CD93

Andere Bezeichnung: CD93 / C1QR1 (CD93 Produkte)

Synonyme: 6030404G09Rik antikoerper, AA145088 antikoerper, AA4.1 antikoerper, AW555904 antikoerper, C1qr1 antikoerper, C1qrp antikoerper, Ly68 antikoerper, C1QR1 antikoerper, C1qR(P) antikoerper, C1qRP antikoerper, CDw93 antikoerper, ECSM3 antikoerper, MXRA4 antikoerper, dJ737E23.1 antikoerper, C1qRp antikoerper, CD93 antigen antikoerper, CD93 molecule antikoerper, Cd93 antikoerper, CD93 antikoerper

Hintergrund: CD93 a cell-surface glycoprotein and type I membrane protein involve in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton.Synonyms: C1q receptor, C1q/MBL/SPA receptor, CDw93, Complement component 1 q subcomponent receptor 1, Complement component C1q receptor, MBL receptor, MXRA4, Matrix-remodeling-associated protein 4, SPA receptor

Gen-ID: 22918

NCBI Accession: NP_036204

UniProt: Q9NPY3