CD93 Antikörper

Produktnummer ABIN111895

Produktdetails anti-CD93 Antikörper

Target: CD93

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD93 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Monoclonal antibody X-2 recognizes a 110 kDa O-sialoglycoprotein on granulocytes, monocytes and endothelial cells. Upon differentiation of cultivated monocytes into macrophages, CDw93 ceases to be expressed on day 5-6, which makes it a very useful marker to differentiate those two cell populations. Antigen distribution on isolated cells: Positive with all granulocytes, with staining increasing transiently upon maturation. Antigen expression is increased by TNFα, GM-CSF, (fMLP) treatment. No effect on superoxide production by binding to neutrophils has been noted. PMA stimulation causes down regulation of the antigen. Positive with monocytes following fMLP, TNF, GM-CSF treatment. Antigen expression decreases on cultured monocytes. Positive on AML cases with monocytic component. Positive on cell lines HL-60, Namalva, K562, THP-1, U937, negative on HMC-1 (human mast cell line). Antigen distribution on tissue sections: Monoclonal antibody X-2 stains epitheloid cells and Rosai-Dorfman cells and most endothelial cells in the synovial membrane of RA patients.

Aufreinigung: Affinity chromatography.

Immunogen: Human monocytes

Klon: X-2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Tested for immunohistochemistry (IHC, Frozen sections: 2 μg/mL (1: 100), Does not react onroutinely processed paraffin sections. Suggested positive control: Human tonsil. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 0.2 mg/mL IgG

Buffer: PBS, pH 7.2 with 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.01 % Thimerosal as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody at -20 °C. Ship at 2-8 °C.

Details zu CD93

Target: CD93

Andere Bezeichnung: CD93 / C1QR1 (CD93 Produkte)

Synonyme: 6030404G09Rik antikoerper, AA145088 antikoerper, AA4.1 antikoerper, AW555904 antikoerper, C1qr1 antikoerper, C1qrp antikoerper, Ly68 antikoerper, C1QR1 antikoerper, C1qR(P) antikoerper, C1qRP antikoerper, CDw93 antikoerper, ECSM3 antikoerper, MXRA4 antikoerper, dJ737E23.1 antikoerper, C1qRp antikoerper, CD93 antigen antikoerper, CD93 molecule antikoerper, Cd93 antikoerper, CD93 antikoerper

Hintergrund: CD93 a cell-surface glycoprotein and type I membrane protein involve in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton.Synonyms: C1q receptor, C1q/MBL/SPA receptor, CDw93, Complement component 1 q subcomponent receptor 1, Complement component C1q receptor, MBL receptor, MXRA4, Matrix-remodeling-associated protein 4, SPA receptor

Gen-ID: 22918

NCBI Accession: NP_036204

UniProt: Q9NPY3