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Granulocytes Antikörper (Biotin)

Dieses Anti--Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von in FACS, IHC (p) und IHC (fro). Geeignet für Ratte.
Produktnummer ABIN111864

Kurzübersicht für Granulocytes Antikörper (Biotin) (ABIN111864)

Target

Granulocytes

Reaktivität

  • 9
  • 6
  • 6
  • 4
Ratte

Wirt

  • 21
Maus

Klonalität

  • 21
Monoklonal

Konjugat

  • 12
  • 5
  • 3
  • 1
Biotin

Applikation

  • 20
  • 5
  • 5
  • 4
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon

RK-4
  • Aufreinigung

    Affinity Chromatography

    Immunogen

    Peritoneal cells.

    Isotyp

    IgG1
  • Applikationshinweise

    Flow cytometry: 1/50-1/100. Immunohistochemistry on frozen sections: 0.75 μg/mL (1/400),does not react on routinely processed paraffin sections. Suggested positive control: Rat spleen, blood smears. Antigen Distribution on Isolated Cells: Only a low percentage of cells from peripheralblood stains positive. Positive on peritoneal cells 18h after thioglycollate injection (95 % granulocytes). Negative on peritoneal cells 4 days after thioglycollate injection. Negative on isolated lymphocytes, monocytes,erythrocytes and platelets. See Table 1. for details.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Rekonstitution

    Restore with 0.5 mL distilled water.

    Konzentration

    0.3 mg/mL IgG

    Buffer

    Stock Solution contains PBS, pH 7.2 with 0.01 % Thimerosal as preservative and 10 mg/mL BSA as stabilizer

    Konservierungsmittel

    Thimerosal (Merthiolate)

    Vorsichtsmaßnahmen

    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
  • Target

    Granulocytes

    Hintergrund

    Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules, Eosinophils have prominent granules that stain readily with the acid dye eosin, and Basophils have prominent granules that stain readily basic (non acidic) dyes.Synonyms: Granulocyte cells
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