SFTPD Antikörper (Lectin Domain)

Produktnummer ABIN111841

Dieses Maus Monoklonal-Antikörper erkennt spezifisch SFTPD in WB, IHC (p), IHC (fro) und EIA. Er zeigt eine Reaktivität gegenüber Human und Ratte.

Kurzübersicht für SFTPD Antikörper (Lectin Domain) (ABIN111841)

Target: SFTPD (Surfactant Protein D (SFTPD))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SFTPD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: VIF11

Produktdetails anti-SFTPD Antikörper

Bindungsspezifität: Lectin Domain

Spezifität: Clone VI F11 is useful for staining rat and human surfactant protein D . Antigen Distribution on Tissues and Cells: Alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on Rat skin, colon, kidney and liver.

Kreuzreaktivität (Details): Species reactivity (tested):Rat and Human. Negative with Pig

Aufreinigung: Affinity Chromatography

Immunogen: Purified Rat Surfactant Protein D (SP-D) from bronchoalveolar lavage. Remarks: The epitope is localized in the lectin domain of SP-D.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen Sections: 0.5 μg/mL (1/800) for Rat Cryosections and10-20 μg/mL (1/20-1/40) for Human Frozen Sections. Immunohistochemistry on Paraffin Sections: 10-20 μg/mL (1/20-1/40) for Rat paraffinsections (microwave pretreatment for antigen retrieval is recommended). Suggested Positive Control: Rat lung. Has been described to work in ELISA and Western blots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore by adding 0.5 mL distilled water (= 0.4 mg/mL Stock Solution).

Konzentration: 0.4 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 0.09 % Sodium Azide as preservative and 5 mg/mL BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C.

Details zu SFTPD

Target: SFTPD (Surfactant Protein D (SFTPD))

Andere Bezeichnung: SFTPD

Hintergrund: Surfactant protein D is a Ca2+ -dependent carbohydrate binding protein with a similar structure as other C-type mammalian lectins such as conglutinin and SP-A. The molecular weight is 620 kD under non reducing conditions and 43 kD under reducing conditions. The function of SP-D is not clearly defined. SP-D enhances the production of oxygen radicals in alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.Synonyms: COLEC7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, Pulmonary surfactant-associated protein D, SFTP4, SP-D

Gen-ID: 25350

NCBI Accession: NP_037010

UniProt: P35248