CD163 Antikörper

Produktnummer ABIN111834

Produktdetails anti-CD163 Antikörper

Target: CD163

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD163 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This antibody recognises the Rat CD163 cell surface glycoprotein, a 175 kDa molecule also known as ED2. It does not react with Human.

Kreuzreaktivität (Details): Species reactivity (tested):Rat.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Rat Spleen cell homogenate. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0Ag 14 mouse myeloma cell line.

Klon: ED2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot. Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/10-1/100 diluted CD163 antibody to label 10^6 cells in 100μL. Immunohistochemistry on Frozen Sections: 1/50-1/100Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval usingpronase prior to staining of paraffin sections. IHC on paraffin-embedded material following PLP fixation(Periodate-lysine-paraformaldehyde) - refer to Whiteland et al (Ref.5. )Histology Positive Control Tissue: liver.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD163

Target: CD163

Andere Bezeichnung: CD163 (CD163 Produkte)

Hintergrund: CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163 is expressed by approximately 50 % of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130

Gen-ID: 9332

NCBI Accession: NP_004235

UniProt: Q86VB7