CD68 Antikörper (Biotin)

Produktnummer ABIN111831

Dieses Anti-CD68-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD68 in FACS. Geeignet für Ratte. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für CD68 Antikörper (Biotin) (ABIN111831)

Target: CD68 (CD68 Molecule (CD68))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD68 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Klon: ED1

Produktdetails anti-CD68 Antikörper (Biotin)

Spezifität: CD68 antibody (Cat#BM4000B) recognises a single chain glycoprotein of 110 kDa that is expressed predominantly on the lysomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages, and weakly be peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 is the rat homologue of human CD68.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Rat spleen cells

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 10 mLof neat antibody to label 10^6 cells in 100 μL. (Results maybe enhanced using membrane permeabilisation).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, pH 7.2 with 0.09 % Sodium Azide as preservative and 1 % BSA as stabiliser

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-CD68 Antikörper (Biotin) (ABIN111831)

Schneider, Meusel, Renker, Bauer, Holzinger, Roeder, Wanner, Gekle, Sauvant: "Low-dose indomethacin after ischemic acute kidney injury prevents downregulation of Oat1/3 and improves renal outcome." in: American journal of physiology. Renal physiology, Vol. 297, Issue 6, pp. F1614-21, (2009) (PubMed).

Details zu CD68

Target: CD68 (CD68 Molecule (CD68))

Andere Bezeichnung: CD68

Hintergrund: The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.Synonyms: Gp110, Macrophage marker, Macrosialin

Gen-ID: 287435

NCBI Accession: NP_001026808

UniProt: Q4FZY1