CRH Antikörper

Produktnummer ABIN111806

Der Maus Monoklonal anti-CRH Antikörper wird verwendet zum Nachweis von CRH in Proben von Human. Er wurde validiert für IHC (p) und EIA.

Kurzübersicht für CRH Antikörper (ABIN111806)

Target: CRH (Corticotropin Releasing Hormone (CRH))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CRH Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Klon: 5D5

Produktdetails anti-CRH Antikörper

Spezifität: Recognises a different epitope than clone 4H9 (BM357). 5D5 mAb binding decreases only partly after CA treatment. It is possible that the antigen determinant is exposed more openly after DEP modification of histidine residues, whereas this determinant becomes barely accesible after lysine modification. Human serum, containing CRF-binding protein, inhibits the binding of CRF to this antibody.Aff. Const.:1.5 x 10-8M for native CRF, 2 x 10-9M for modified CRF

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Synthetic Human Corticotropin Releasing Factor

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: High binding constant allows this antibody to be used in a sandwich ELISA withclone number 4H9 (BM357), Sensitivity: 25 pg/mL. Immunohistochemistry on frozen sections: A 4 μg/mL solution has worked well on paraffinsections, after microwave pre-treatment.0.1 % trypsin digestion may also be necessary,The detection system used was avidin-biotin. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CRH

Target: CRH (Corticotropin Releasing Hormone (CRH))

Andere Bezeichnung: Corticoliberin

Hintergrund: CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).Synonyms: CRF, CRH, Corticotropin-Releasing Factor, Corticotropin-Releasing Hormone

Gen-ID: 1392

NCBI Accession: NP_000747

UniProt: P06850

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour